Defining the Teratoma as a Model for Multilineage Human

Leave Comment

Defining the Teratoma as a Model for Multi-lineage

We propose that the teratoma, a recognized standard for validating pluripotency in stem cells, could be a promising platform for studying human developmental processes. Performing single-cell RNA sequencing (RNA-seq) of 179,632 cells across 23 teratomas from 4 cell lines, we found that teratomas rep

Get price

Defining the Teratoma as a Model for Multi-lineage

2020-11-25  To establish the utility of the teratoma system as a model for human development, we next performed a single-cell genetic knockout screen using CRISPR-Cas9. Specifically, we focused on 24 major organ/lineage specification genes that are embryonic lethal upon knockout in mice (Table S4A). Studying the effects of these genes using cell lines or ...

Get price

Defining the Teratoma as a Model for Multi-lineage

2021-5-30  Resource Defining the Teratoma as a Model for Multi-lineage Human Development Daniella McDonald,1,2,6 Yan Wu,1,6 Amir Dailamy,1 Justin Tat,3 Udit Parekh,5 Dongxin Zhao,1 Michael Hu,1 Ann Tipps,4 Kun Zhang,1,2, *and Prashant Mali1,2,7, 1Department of Bioengineering, University of California, San Diego, San Diego, CA 92093, USA 2Biomedical Sciences Graduate Program,

Get price

Defining the Teratoma as a Model for Multi-lineage

Defining the Teratoma as a Model for Multi-lineage Human Development Author links open overlay panel Daniella McDonald 1 2 6 Yan Wu 1 6 Amir Dailamy 1 Justin Tat 3 Udit Parekh 5 Dongxin Zhao 1 Michael Hu 1 Ann Tipps 4 Kun Zhang 1 2 Prashant Mali 1 2 7

Get price

Defining the Teratoma as a Model for Multi-lineage

2020-11-21  Resource Defining the Teratoma as a Model for Multi-lineage Human Development Daniella McDonald,1,2,6 Yan Wu,1,6 Amir Dailamy,1 Justin Tat,3 Udit Parekh,5 Dongxin Zhao,1 Michael Hu,1 Ann Tipps,4 Kun Zhang,1,2, *and Prashant Mali1,2,7, 1Department of Bioengineering, University of California, San Diego, San Diego, CA 92093, USA 2Biomedical Sciences Graduate Program,

Get price

Defining the Teratoma as a Model for Multi-lineage

2020-11-4  The teratoma is characterized as a model for multi-lineage human development with cell types represented across all three germ layers and utilized to enable assaying of the effects of genetic perturbations simultaneously across multiple cell types, and a molecular sculpting strategy is presented to enrich for specific tissues.

Get price

Defining The Teratoma as a Model for Multi-Lineage

Defining The Teratoma as a Model for Multi-Lineage Human Development. No data is associated with this publication. We propose that the teratoma, a recognized standard for validating pluripotency in stem cells, could be a promising platform for studying human developmental processes. Performing single cell RNA-seq of 179,632 cells across 23 ...

Get price

Defining the Teratoma as a Model for Multi-lineage

As a new in vivo model of human multilineage development, teratomas have similarities and differences with organoids (McDonald et al., 2020). Both organoid and teratoma are derived from PSCs. ...

Get price

Defining the Teratoma as a Model for Multi-lineage

2020-11-4  Search worldwide, life-sciences literature Search. Advanced Search Coronavirus articles and preprints Search examples: "breast cancer" Smith J"breast cancer" Smith J

Get price

Cell发现研究人体发育的新工具:让人着迷的“巨大肿瘤” - 生物通

2020-11-5  Defining the Teratoma as a Model for Multi-lineage Human Development 相关新闻 生物通微信公众号 生物通新浪微博 微信 新浪微博 我要投稿 搜索 国际 国内 人物 产业 热点 科普 热搜: 畸胎瘤发育研究molecularly sculpt ...

Get price

Defining The Teratoma as a Model for Multi-Lineage

Defining The Teratoma as a Model for Multi-Lineage Human Development. No data is associated with this publication. We propose that the teratoma, a recognized standard for validating pluripotency in stem cells, could be a promising platform for studying human developmental processes. Performing single cell RNA-seq of 179,632 cells across 23 ...

Get price

GEO Accession viewer

2020-11-4  Title. Defining the Teratoma as a Model for Multi-Lineage Human Development. Organism. Homo sapiens. Experiment type. Expression profiling by high throughput sequencing. Other. Summary. Studying early human developmental processes requires access to a model system that is easy to perturb, either genetically or chemically, genetically tractable ...

Get price

科学家发现畸胎瘤可用于探究人多谱系发育—小柯 ...

2020-11-4  Title: Defining the Teratoma as a Model for Multi-lineage Human Development Author: Daniella McDonald, Yan Wu, Amir Dailamy, Justin Tat, Udit Parekh, Dongxin Zhao, Michael Hu, Ann Tipps, Kun Zhang ...

Get price

Cell:畸胎瘤不仅是瘤,还有望应用于多谱系发育建模!_细胞

2020-11-12  2020年11月4日,《Cell》杂志在线发表了美国加州大学圣地亚哥分校Prashant Mali和张鹍团队名为“Definingthe Teratoma as a Model for Multi-lineage Human Development”的合作研究。在此研究中,他们探讨了畸胎瘤作为人多谱系发育模型的潜力。 DOI: 10.1016

Get price

2021-03-01文献阅读 - 简书

2021-3-1  Defining the Teratoma as a Model for Multi-lineage Human Development. 数据: 179,632 cells across 23 teratomas from 4 cell lines. 细胞类型: approximately 20 cell types across all 3 germ layers. 观点:单细胞实验20种细胞类型,和类器官和胚胎的数据做比较,利用CRISPR-Cas9做敲除扫描。. 利用miRNA调节的 ...

Get price

New Insights Into Human Development From “Monster

2020-11-4  Reference: “Defining the Teratoma as a Model for Multi-lineage Human Development” by Daniella McDonald, Yan Wu, Amir Dailamy, Justin Tat, Udit Parekh, Dongxin Zhao, Michael Hu, Ann Tipps, Kun Zhang and Prashant Mali, 4 November

Get price

Zane_bio - 简书

2018-7-19  Defining the Teratoma as a Model for Multi-lineage Human Development 数据:... Jay Shendure课题组预发表的课题 文献简介 基因调控是反式调控元件(TF)结合到顺式调控元件(enhancer)的过程。. 该课题组设计了... Phenotypic variation of transcriptomic cell types in mouse motor cortex ... INTRODUCTION ...

Get price

Teratoma formation of human embryonic stem cells in

Teratoma formation in mice is today the most stringent test for pluripotency that is available for human pluripotent cells, as chimera formation and tetraploid complementation cannot be performed with human cells. The teratoma assay could also be applied for assessing the safety of human pluripotent

Get price

Research - University of California, San Diego

2021-5-30  " Defining the Teratoma as a Model for Multi-Lineage Human Development," Cell (2020). " Immune-orthogonal orthologues of AAV capsids and of Cas9 circumvent the immune response to the administration of gene therapy," Nature Biomedical Engineering (2019).

Get price

组内活动 - 江苏大学再生医学研究院

Defining the Teratoma as a Model for Multi-lineage Human Development Alpha-Ketoglutarate, an Endogenous Metabolite, Extends Lifespan and Compresses Morbidity in Aging Mice Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair

Get price

Defining The Teratoma as a Model for Multi-Lineage

Defining The Teratoma as a Model for Multi-Lineage Human Development. No data is associated with this publication. We propose that the teratoma, a recognized standard for validating pluripotency in stem cells, could be a promising platform for studying human developmental processes. Performing single cell RNA-seq of 179,632 cells across 23 ...

Get price

科学家发现畸胎瘤可用于探究人多谱系发育—小柯 ...

2020-11-4  Title: Defining the Teratoma as a Model for Multi-lineage Human Development Author: Daniella McDonald, Yan Wu, Amir Dailamy, Justin Tat, Udit Parekh, Dongxin Zhao, Michael Hu, Ann Tipps, Kun Zhang ...

Get price

Research - University of California, San Diego

2021-5-30  " Defining the Teratoma as a Model for Multi-Lineage Human Development," Cell (2020). " Immune-orthogonal orthologues of AAV capsids and of Cas9 circumvent the immune response to the administration of gene therapy," Nature Biomedical Engineering (2019).

Get price

Zane_bio - 简书

2018-7-19  Defining the Teratoma as a Model for Multi-lineage Human Development 数据:... Jay Shendure课题组预发表的课题 文献简介 基因调控是反式调控元件(TF)结合到顺式调控元件(enhancer)的过程。. 该课题组设计了... Phenotypic variation of transcriptomic cell types in mouse motor cortex ... INTRODUCTION ...

Get price

Weekly reads: Alzheimer's, dinosaur brains, teratoma ...

2020-11-8  Defining the Teratoma as a Model for Multi-lineage Human Development, Cell. This resource article reports sc-RNA-Seq data from teratoma, which I don’t recall seeing before. Blast from the past From boobs to baldness: stem cells go cosmetic

Get price

Is teratoma formation in stem cell research a ...

2012-11-22  human embryonic stem cells, pluripotency, teratoma. Teratoma formation . in vivo. is one of the most important approaches used to determine the developmental potential of pluripotent stem cells (SC) such as ESC and to demonstrate the ability of newly derived cell lines to differentiate as part of their characterization process (Thomson. et al.

Get price

Teratomas could provide a new glance at human

2020-11-5  Teratomas could provide a new glance at human development. Finding just the right model to study human development--from the early embryonic stage onward--has been a challenge for scientists over the last decade. Now, bioengineers at the

Get price

Human Induced Pluripotent Stem Cells Develop

6 Prokhorova TA, Harkness LM, Frandsen U et al. Teratoma formation by human embryonic stem cells is site-dependent and enhanced by the presence of matrigel. Stem Cells Dev 2009;18:47–54. 7 Muller FJ, Goldmann J, Loser P et al. A call to standardize teratoma assays used to define human pluripotent cell lines. Cell Stem Cell 2010;6:412–414.

Get price

Establishment of human iPSC-based models for the study

2016-2-22  Directly addressing the lack of model for studying driver mutations, the Tabar laboratory first reported on the use of NPCs differentiated from human ESCs for studying the role that histone 3.3 ...

Get price

Murine embryonic stem cells as a model for human

2009-7-25  Over the last several decades, murine embryonic stem cells (mESCs) have been used as a model for human embryonic stem cell (hESC) research. The relevance of this approach has not yet been proven. There is a great deal of evidence that is indicative of substantial differences between these two cell types. An analysis of the literature shows that the differences concern ESC proliferation, self ...

Get price
Contact us

Submit online

Please leave a message below and we will contact you as soon as possible.